photo H. Cottet 0622

Hervé Cottet



Institut des Biomolécules Max Mousseron


Université de Montpellier 2,

Place Eugène Bataillon,  Case courrier 1706

34095 Montpellier Cedex 5, France


Phone : (+33) 4 67 14 34 27

Fax : (+33) 4 67 63 10 46

Email :


  Research Interests

Electrokinetic separation techniques (free solution, gel, micellar, isoelectric focusing, isotachophoretic, 2D and frontal modes)
Taylor Dispersion Analysis 

Characterization of polyelectrolytes, biopolymers and colloïds
Study of biomolecule interactions (stoichiometry, affinity constants)
Electrophoretic mobility modelling
Capillary coatings (SMIL)
Characterization and study of bacteria
Dendrigraft Poly-L-lysine

1st European Workshop on Taylor Dispersion Analysis
(held on monday june 2, 2014 in Montpellier)

2nd European Workshop on Taylor Dispersion Analysis
(held on monday june 6, 2016 in Copenhagen)

Curriculum Vitae




Industrial and academic programs



  Recent papers (2013-2016): complete list here

P35. Interactions between oppositely charged polyelectrolytes by isothermal titration calorimetry: effect of ionic strength and charge density. F. Lounis, J. Chamieh, L. Leclercq, P. Gonzalez, H. Cottet, J. Phys. Chem., 2017, in revision.

P34. Analysis and characterization of aluminum chlorohydrate oligocations by capillary electrophoresis. N. Ouadah, C. Moire, J.-F. Kuntz, F. Brothier, H. Cottet, J. Chromatogr. A., 2017, accepted.

P33. The effect of molar mass and charge density on the formation of complexes between oppositely charged polyelectrolytes. F. Lounis, J. Chamieh, L. Leclercq, P. Gonzalez, H. Cottet, Polymers, 2017, accepted.

P32. Determination of the Distributions of the Degrees of Acetylation of Chitosan. J. Thevarajah, M. Van Leeuwen, H. Cottet, P. Castignolles, M. Gaborieau, International Journal of Biological Macromolecules, 2017, 95, 40-48.

P31. On the ionic strength dependence of the electrophoretic mobility: from 2D to 3D slope-plots. H. Cottet, H. Wu, S. A. Allison, Electrophoresis, 2016, DOI: 10.1002/elps.201600329.

P30. Study of the interactions between oppositely and variously charged polyelectrolytes by frontal analysis continuous capillary electrophoresis. F. M. Lounis, J. Chamieh, L. Leclercq, P. Gonzalez, H. Cottet, Soft Matter, 2016, 12, 9728 - 9737.

P29. Advanced  portrayal  of  SMIL  coating  by  allying  CZE  performance  with  in-capillary topographic and charge-related surface characterization. L. G. Stock, M. Leitner, L. Traxler, K. Bonazza, L. Leclercq, H. Cottet, G. Friedbacher, A. Ebner, H. Stutz, Analytica Chimica Acta, 2016, 930, 39-48.

P28. What is the contribution of counter-ions in the absolute molar mass of polyelectrolytes determined by SEC/MALLS ? P. Gonzalez, L. Leclercq, H. Cottet, Macromol. Chem. Phys., 2016, 217, 2654–2659.

P27. Hydrodynamic size characterization of a self-emulsifying lipid pharmaceutical excipient by Taylor dispersion analysis with fluorescent detection. J. Chamieh, V. Jannin, F. Demarne, H. Cottet, Int. J. Pharm., 2016, 513, 262-269.

P26. Mapping Molecular Adhesion Sites inside SMIL coated Capillaries using Atomic Force Microscopy Recognition Imaging. M. Leitner, L. G. Stock, L. Traxler, L. Leclercq, K. Bonazza, G. Friedbacher, H. Cottet, H. E. Stutz, A. Ebner, Analytica Chimica Acta, 2016, 930, 39-48.

P25. Prediction of polyelectrolyte complex stoichiometry for highly hydrophilic polyelectrolytes. F. Lounis, J. Chamieh, P. Gonzalez, H. Cottet, L. Leclercq, Macromolecules, 2016, 49, 3881–3888.

P24. A new robust estimator of polydispersity from dynamic light scattering data. V. Roger, H. Cottet, L. Cipelletti, Anal. Chem., 2016, 88, 2630-2636.

P23. Quantifying the heterogeneity of chemical structures in complex charged polymers through the dispersity of their distributions of electrophoretic mobilities or of compositions. J. J. Thevarajah, A. T. Sutton, A. R. Maniego, E. G. Whitty, S. Harrison, H. Cottet, P. Castignolles, M. Gaborieau, Anal. Chem., 2016, 88, 1674-1681.

P22. Influence of the ionic strength of acidic background electrolytes on the separation of proteins by capillary electrophoresis. S. Bekri, L. Leclercq, H. Cottet, J. Chromatogr. A, 2016, 1432, 145-151.

P21. Monitoring biopolymer degradation by Taylor dispersion analysis. J. Chamieh, J.-P. Biron, L; Cipelletti, H. Cottet, Biomacromolecules, 2015, 16, 3945-3951.

P20. Size-based characterization of nanoparticle mixtures by the inline coupling of Capillary Electrophoresis to Taylor Dispersion Analysis. F. Oukacine, A. Morel, H. Cottet J. Chromatogr. A. 2015, 1426,  220-225.

P19. Fast Characterization of Polyplexes by Taylor Dispersion Analysis. L. Leclercq, S. Reinhard, J. Chamieh, E. Wagner, H. Cottet, Macromolecules, 2015, 48, 7216-7221.

P18. Measuring arbitrary diffusion coefficient distributions of nano-objects by Taylor dispersion analysis. L. Cipelletti, J.-P. Biron, M. Martin, H. Cottet, Anal. Chem., 2015, 87, 8489–8496.

P17. Size characterization of commercial micelles and microemulsions by Taylor dispersion analysis. J. Chamieh, F. Davanier, V. Jannin, F. Demarne, H. Cottet, Int. J. Pharm., 2015, 492, 46-54.

P16. Taking advantage of electric field induced bacterial aggregation for the study of interactions between bacteria and macromolecules by capillary electrophoresis. N. Sisavath, P. Got, G. M. Charrière, D. Destoumieux-Garzon, H. Cottet, Anal. Chem., 2015, 87, 6761-6768.

P15. Polyelectrolyte Multilayer Coatings for the Separation of Proteins by Capillary Electrophoresis: Influence of Polyelectrolyte Nature and Multilayer Crosslinking. S. Bekri, L. Leclercq, H. Cottet, J. Chromatogr. A., 2015, 1399, 80-87.

P14. Quantitative analysis in capillary electrophoresis: transformation of raw electropherograms into continuous distributions. J. Chamieh, M. Martin, H. Cottet, Anal.Chem., 2015, 87, 1050-1057.

P13. Generalized polymer effective charge measurement by capillary isotachophoresis. J. Chamieh, D. Koval, A. Besson, V. Kašička, H. Cottet, J. Chromatogr. A, 20141370, 255-262.

P12. Physico-chemical characterization of polymeric micelles loaded with platinum derivatives by capillary electrophoresis and related methods. F. Oukacine, S. Bernard, I. Bobe, H. Cottet, J. Control. Release, 2014, 196, 139-145.

P11. Investigating the Influence of Phosphate Ions on Poly-L-lysine Conformations by Taylor Dispersion Analysis. X. Jin, L. Leclercq, N. Sisavath, H. Cottet, Macromolecules, 2014, 47, 5320–5327.

P10. Polydispersity analysis of Taylor dispersion data: the cumulant method. L. Cipelletti, J.-P. Biron, M. Martin, H. Cottet, Anal. Chem. 2014, 86, 6471–6478.

P9.  On the optimization of operating conditions for Taylor Dispersion Analysis of Mixtures. H. Cottet, J. P. Biron, M. Martin, Analyst, 2014, 139, 3552-3562.

P8.  Effect of dendrimer generation on the interactions between human serum albumin and dendrigraft polylysines. N. Sisavath, T. Le Saux, L. Leclercq, H. Cottet, Langmuir, 2014, 30, 4450–4457.

P7.  Correlation of length of linear oligo(ethanamino) amides with gene transfer and cytotoxicity. C. Scholz, P. Kos, L. Leclercq, X. Jin, H. Cottet, E. Wagner, ChemMedChem, 2014, 9, 2104 – 2110.

P6.  Determination of polymer log D distributions by micellar and microemulsion electrokinetic chromatography. X.Y. Jin, L. Leclercq, H. Cottet, J. Chromatogr. A, 2013, 1318, 244-250.

P5.  Phosphonated Oligoallylamine : Synthesis, Characterization in Water and Development of Layer by Layer Assembly. C. Negrell-Guirao, F. Carosio, B. Boutevin, H. Cottet, C. Loubat, Journal of Polymer Science Part B: Polymer Physics, 2013, 51, 16, 1244–1251.

P4.  Size and Charge Characterization of Polymeric Drug Delivery Systems by Taylor Dispersion Analysis and Capillary Electrophoresis. A. Ibrahim, R. Meyrueix, G. Pouliquen, Y. P. Chan, H. Cottet, Anal. Bioanal. Chem., 2013, 405, 16, 5369–5379.

P3.  Study of interactions between oppositely charged dendrigraft poly-L-lysine and human serum albumin by frontal analysis continuous capillary electrophoresis and fluorescence spectroscopy. N. Sisavath, L. Leclercq, T. Le Saux, F. Oukacine, H. Cottet, J. Chromatogr. A, 2013, 1289, 127-132.

P2.  Monitoring surface functionalization of dendrigraft poly-L-lysines via click chemistry by capillary electrophoresis and Taylor dispersion Analysis. T. Liu, F. Oukacine, H. Collet, A. Commeyras, L. Vial, H. Cottet. J. Chromatogr. A, 2013, 1273, 111-116.

P1.  Effective Charge Determination of Dendrigraft poly-L-Lysine by Capillary Isotachophoresis. A. Ibrahim, D. Koval, C. Faye, H. Cottet. Macromolecules, 2013, 46, 533-540.


  Awardee 2016 of the French Scholar Series (Peter Wall Institute for Advanced Studies and Frenh Consulate Vancouver)

    Junior Member of the Institut Universitaire de France (IUF), 2011-2016.

    Award "Chercheur d'Avenir", Région Languedoc-Roussillon, 2010-2013.

    Award from the French Chemical Society (Analytical Chemistry Division), 2006.

    Lauréat ACI jeunes chercheurs (French National Research Agency), 2004-2008.


Last update: Feb. 2, 2017

Taylor dispersion analysis, capillary electrophoresis, polyelectrolyte complexes, characterization of polymers, nanoparticles, dendrigraft polymers, dendrimers, analysis of bacteria; analysis of polymers, drug delivery systems. Expertise en électrophorèse capillaire. Consultance en électrophorèse capillaire. Expertise and consultancy in capillary electrophoresis and Taylor dispersion analysis.